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Figure 3 | Molecular Cancer

Figure 3

From: SAP domain-dependent Mkl1 signaling stimulates proliferation and cell migration by induction of a distinct gene set indicative of poor prognosis in breast cancer patients

Figure 3

The different HC11 cell strains proliferate at different rates and show distinct migration behaviors. (A) Immunoblot with mAb65F13 of Mkl1 proteins in whole-cell extracts from the empty vector-, FL-, mutB1- or ΔSAP-transfected HC11 strains. Anti-Gapdh served as loading control. Endogenous Mkl1 protein was below the detection limit in empty vector cells. (B) SAP-dependent proliferation of HC11 mammary epithelial cells. Proliferation rates of the four HC11 cell strains were assessed by BrdU incorporation into newly synthesized DNA immediately after plating (0 h) as well as at 24, 48, 72 and 96 h. Means ± SD from three independent experiments and significant differences to the HC11-ΔSAP cells, ***P < 0.001, **P < 0.01, *P < 0.05 are shown. (C) SAP-dependent migration of HC11 mammary epithelial cells. Cell migration of the four HC11 strains was evaluated by Transwell migration assay using filters with 8 μm pore size. Quantification of the cell migration was measured by the area on the lower side of the filter covered with cells. Above the bar graph, a photo of fixed and stained cells seeded in parallel in a 24-well plate is shown as a seeding control, and representative photos of fixed and stained cells of each of the cell strains that have migrated to the lower side of the filter, are shown below (bar, 200 μm). Data and statistical significance are expressed as in (B).

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