Figure 2

MC3 induced cell apoptosis in Panc1. The cells were incubated with various concentrations of MC3 for 24 h (A) and 48 h (B), and analyzed by FACS using annexin v/PI staining. (C) S and G2/M phase cell arrests occurred 24 h after treatment in Panc1. The relative DNA content was analyzed by FACS using PI staining. The percentage of cells was determined by using trypan blue assay. (D) MC3 prevented colony formation in Panc1. Panc1 cells were treated with increasing concentrations of MC3 for 24 h, re-plated at 2000 cells/well in a new 6-well plate and detected with crystal violet after 3 weeks cultivation. (E) MC3 inhibited cell migration in wound healing assay. Panc1 cells were incubated with DMF as mock or MC3 (1.25 μM) under optimized condition as described in experimental section, and photos were taken at 0 h, 24 h and 48 h.