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Figure 3 | Molecular Cancer

Figure 3

From: A TrxR inhibiting gold(I) NHC complex induces apoptosis through ASK1-p38-MAPK signaling in pancreatic cancer cells

Figure 3

MC3 interfered cellular redox homeostasis. MC3 promoted ROS formation in time- (A) and concentration (B)-dependent manner. Panc1 cells were incubated with MC3 as indicated and ROS formation was measured using DHE. Data were normalized to the value of DMF-treated cells. (C) MC3 reduced membrane potential. Panc1 cells were treated with MC3 5 μM at indicated time points and collected for the measurement as described in the experimental section. The data show a clear reduction in the ratio of red population defined as R2 to green population defined as R3. DMF was used as mock and CCCP (carbonyl cyanide 3-chlorophenylhydrazone) as the positive control. Data were normalized to the value of non-treated cells. The raw data of mock and MC3 treatment for 3 h are depicted in an inset picture. (D) MC3 inhibited Bcl-xL activity. The amount of Bcl-xL was determined by FACS using Alexa Flur® 488-conjugated Bcl-xL antibody in 24 h MC3-treated Panc1 cells. DMF was used as mock.

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