Figure 5

Opposite effects of ROS scavenger and p38i against MC3. (A) ROS scavengers and p38i rescued MC3-induced cell apoptosis in 24 h treatment. Panc1 cells were incubated with 5 μM MC3, 10 μM p38i, 10 mM NAC or 5 mM Glu or in combination as indicated. The cell apoptosis was analyzed by using annexin v/PI staining. Immunoblots showing: (B) p38i prevented PARP cleavage caused by MC3; and (C) reduced phospho-p38 levels and inhibition of PARP cleavage after co-treatment with ROS scavengers. Panc1 cells were incubated with inhibitors or MC3 or in combination for 24 h as indicated. Vinculin was used as loading control. (D) Alternation of ROS production in combination of MC3 with inhibitors. ROS formation was analyzed by FACS in Panc1 cells treated with various compounds or compounds combination for 24 h. The mean values were listed. (E) Glu compensated MC3-mediated cell cycle arrest after 24 h treatment. The densitometric analyses of corresponding immunoblots are provided in supporting information.