Figure 2

KB9520 significantly increases cisplatin/pemetrexed effects in vitro and in vivo . A) Percentage of viable REN cells exposed for 24 hours to 10 nM KB9520 alone or in combination with cisplatin (100 μM) and pemetrexed (22 μM), versus untreated cells. Graph is representative of three independent experiments. Each bar represents mean ± s.d. *p ≤ 0.05. B) and C) Box plots of the 4 different treatment groups (10 mice/group) showing in vivo mean tumor growth (B) and mean tumor growth inhibition (C) evaluated after 21 days of treatment. The thick segments represent the medians while the upper and lower borders of each rectangle represent the quartiles. Bars show minimum and maximum values for each group, outliers are identified by a small circle. Kruskal-Wallis rank sum test (in B) confirms that combination treatment significantly reduced tumor growth, compared with control or single treatments.