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Figure 2 | Molecular Cancer

Figure 2

From: microRNA regulation of mammalian target of rapamycin expression and activity controls estrogen receptor function and RAD001 sensitivity

Figure 2

Overexpression of miR-155 selectively alters estrogen stimulated gene expression of ER-regulated genes in vitro . (A-B) Gene panel array for ER regulated and cancer associated genes, n = 2. (A) Down regulated genes and (B) up regulated genes (logarithmic scale) in MCF-7-miR-155 cells compared to vector control. Results represent average fold change ± SEM. (C-F) QPCR was performed for genes, n = 5 (C) PgR, (D) SDF-1, (E) BCL2, and (F) SERPINA3. Cells were grown in 5% CS phenol free DMEM for 48 hours before treatment with DMSO or E2 (1nM) for 24 hours. Cycle number was normalized to beta actin and MCF-7-vector control cells treated with DMSO scaled to 1. Bars represent average fold change ± SEM. * p < 0.05; ** p < 0.01; *** p < 0.001.

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Molecular Cancer

ISSN: 1476-4598