Figure 1

Preservation of cell proliferation by BLF501 in small intestinal crypts of mice treated with a single administration of DXR. Immunofluorescence assay for BrdU-positive proliferating cells was performed. DXR-induced decrease of proliferation rate apoptosis was observed at the three- to six-cell positions within crypts. Positive cells count was performed. (A) UNTR, untreated; (B) BLF501 25 μg/kg; (C) DXR 48 h; (D) DXR 72 h; (E) DXR + BLF501 25 μg/kg 48 h; (F) DXR + BLF501 25 μg/kg 72 h. DXR + BLF501 25 μg/kg 48 h vs DXR 48 h, ** p = 0.004; DXR + BLF501 25 μg/kg 72 h vs DXR 72 h, * p = 0.0282. Experiments were performed in triplicate.