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Figure 5 | Molecular Cancer

Figure 5

From: Sodium glucose cotransporter 1 ligand BLF501 as a novel tool for management of gastrointestinal mucositis

Figure 5

Preservation of tight junction and adherence junction integrity by BLF501 in mice repeatedly injected with DXR and 5FU, stabilizing ZO-1 and beta-catenin expression and distribution. ZO-1 immunofluorescence assay: (A) UNTR, untreated; (B) DXR + 5-FU; (C) BLF501 25 μg/kg; (D) DXR + 5-FU + BLF501 2.5 μg/kg; (E) DXR + 5-FU + BLF501 25 μg/kg. Magnification 60X. Immunohistochemical analysis of beta-catenin: (F) UNTR, untreated; (G) DXR + 5-FU; (H) DXR + 5-FU + BLF501 2.5 μg/kg; (I) DXR + 5-FU + BLF501 25 μg/kg; (J) BLF501 25 μg/kg. Magnification 40X. Western blot assay for epithelial damage markers caspase 3 (K), ERM complex (L), and GAPDH as housekeeping protein (M). Experiments were performed in triplicate.

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