Figure 4
From: Targeting DNA-PKcs and telomerase in brain tumour cells
Role of DNA-PKcs in MST-312-induced double strand breaks in brain tumour cells. (A) Extent of DSBs was measured as γH2AX foci formation following 1.0 μM MST-312 and/or 10 μM NU7026 treatment. There was significant increase in the distribution of cells positive for γH2AX (damage) in MST-312 treated cells. Following 24 hours recovery (repair) period, the γH2AX level returned to basal level only in DNA-PKcs proficient cells. (B) Protein expression studies following (1.0 μM) MST-312 treatment in MO59K cells. Data represent mean ± SE from 3 independent experiments. *P-value <0.05 when compared to untreated controls. (C-D) Effects of combined treatment of MST-312 and NU7026 in U2OS cells. (C) Level of γH2AX foci positive cells following treatment with 1.0 μM MST-312 and 10 μM NU7026 in telomerase negative U2OS cells. (D) DNA-PKcs status following 48 hours treatment with 1.0 μM MST-312 and/or 10 μM NU7026.