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Figure 1 | Molecular Cancer

Figure 1

From: Cyr61 promotes epithelial-mesenchymal transition and tumor metastasis of osteosarcoma by Raf-1/MEK/ERK/Elk-1/TWIST-1 signaling pathway

Figure 1

Cyr61 is associated with the cell migration phenotype. (A) MG63 cells and its sublines were selected according to description in the Methods section, and cell migration was examined using the Transwell assay. (B and C) Total mRNA and proteins were extracted from the MG63 cells and its sublines, and Cyr61 expression was detected using qRT-PCR and western blotting. (D and E) Cells were incubated with Cyr61 (1-30 ng/mL) for 24 h, and cell migration was measured using the Transwell and wound-healing assays. (F) MG63 cells were pretreated with anti-Cyr61 mAb (25-200 ng/mL) for 24 h. Cell migration was measured after 24 h using the Transwell assay. The results are expressed as the mean ± SEM of triplicate samples. *P <0.05 compared with the control group.

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