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Figure 2 | Molecular Cancer

Figure 2

From: Cyr61 promotes epithelial-mesenchymal transition and tumor metastasis of osteosarcoma by Raf-1/MEK/ERK/Elk-1/TWIST-1 signaling pathway

Figure 2

Cyr61 regulates EMT markers and promotes mesenchymal transformation. (A and B) Total mRNA and proteins were extracted from the MG63 cells and its sublines, and the expression levels of TWIST-1, N-cadherin and E-cadherin were detected using qRT-PCR and western blotting. (C) Phase-contrast images of the MG63 cells and their sublines were photographed. The subconfluent cultures were shown to indicate the morphological differences between MG63 and the sublines. (D and E) Cells were incubated with Cyr61 (1-30 ng/mL) for 24 h, total mRNA and proteins were extracted from the MG63 cells, and the expression levels of TWIST-1, N-cadherin, and E-cadherin were detected using qRT-PCR and western blotting. (F and G) Cells were incubated with Cyr61 (10 ng/mL) for different time (0-24 h), total mRNA and proteins were extracted from the MG63 cells, and the expression levels of TWIST-1, N-cadherin and E-cadherin were detected using qRT-PCR and western blotting. (H) MG63 cells were transfected with TWIST-1 shRNA or control shRNA for 24 h, followed by treatment with Cyr61 (10 ng/mL) for 24 h, and cell migration was analyzed using the Transwell assay. The results are expressed as the mean ± SEM of triplicate samples. *P <0.05 compared with the control group, and #P <0.05 compared with Cyr61 treatment.

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