Figure 2
From: SPARC mediates metastatic cooperation between CSC and non-CSC prostate cancer cell subpopulations
SPARC is the secreted protein most abundantly produced by S cells relative to M cells. (a) Schematic depiction of the procedure used to identify the proteins present in M-CM and S-CM using SILAC labeling. (b) Gene Ontology and Uniprot subcellular localization predictions for the differentially secreted proteins. (c) Gene Ontology biological process predictions for the differentially secreted proteins. S > M: proteins with H/L ≥2. S < M: proteins with H/L ≤ -2. (d) Western blotting analysis of cell extracts and conditioned medium confirms the differential expression of SPARC and PAI-1 between S and M cells. Tubulin signal was used as a protein loading and transfer control for cell lysates, and Ponceau red staining for conditioned medium.