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Figure 1 | Molecular Cancer

Figure 1

From: Direct but not indirect co-culture with osteogenically differentiated human bone marrow stromal cells increases RANKL/OPG ratio in human breast cancer cells generating bone metastases

Figure 1

Gene expression of BOKL and BMSCs from different patients cultured for 3 days in direct contact. A) Expression of genes related to RANK/RANKL/OPG pathway and CDH11 in BOKL after direct co-culture with osteo-differentiated BMSCs (BOKL co-culture BMSC) compared with BOKL cultured in medium conditioned by the same osteo-differentiated BMSCs (BOKL CM BMSC), with BOKL after direct co-culture with fibroblasts (BOKL co-culture MRC-5) and with BOKL cultured in medium conditioned by the same fibroblasts (BOKL CM MRC-5). *: p < 0.05, **: p < 0.01 and ***: p < 0.001. Fold changes were calculated respective to control 1 or 2 (expression set to 1, dotted line). Control 1: BOKL grown in GM and subjected to enzymatic digestion and FACS sorting, used for BOKL from both direct co-cultures, control 2: BOKL grown in GM, used for BOKL in both CMs. B) RANKL/OPG ratio, for BOKL after direct co-culture with BMSCs and fibroblasts (BOKL co-culture BMSC and BOKL co-culture MRC-5) and in BOKL cultured in medium conditioned by BMSCs and fibroblasts (BOKL CM BMSC and BOKL CM MRC-5). **: p < 0.01. C) Gene expression of osteocalcin (OCN), osteopontin (OPN), RUNX-2 and RANK in BMSCs from three different patients (Pt1, Pt2, Pt3) after direct co-culture with BOKL. Fold changes were calculated respective to the same BMSCs before co-culture (expression value set to 1, dotted line). D) RANKL expression of BMSCs before (BMSC CTRL) and after direct co-culture (BMSCs co-culture BOKL), relative to three different patients, compared to that of osteoblasts. Fold changes were calculated respective to fibroblasts (expression set to 1, dotted line). All error bars represent standard deviations of at least three different experiments.

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