Characterization of the bone-like microenvironment. Characterization of the osteo-differentiation level of BMSCs from three different patients cultured for 14 days in osteogenic medium (DMEM additioned with 10% FBS, 1% HEPES, 1% pen strep, 15 mM ascorbic acid, 10 mM β - glycerophosphate, 10 nM dexamethasone, 10nM cholecalciferol). A) Alizarin red staining for the three different BMSCs populations; B) Expression of typical osteoblastic markers, normalized to MRC-5 expression (set to 1) and also quantified in RNA deriving directly from human bone samples (osteoblasts). *: p < 0.05, ***: p < 0.001 as compared to osteoblasts expression. All error bars represent standard deviations of at least three different experiments.