Figure 5
From: ADAM17 mediates OSCC development in an orthotopic murine model
Tumors induced by injection of SCC-9 cells overexpressing ADAM17 (AD17-HA) have increased Erk phosphorylation. A: The immunoblotting indicates the expression of ADAM17-HA (anti-HA), total Erk (anti-Erk), phosphorylated Erk (anti-phospho Erk) and as loading control (anti-GAPDH). B: Phosphorylation levels were calculated by band intensity using ImageJ software (n = 2, Fisher’s exact test p = 0.0034). C: Knockdown of ADAM17 expression by shRNA in A431 cell line. Relative ADAM17 mRNA levels comparing shRNA and scrambled and mock control-treated cells were determined by quantitative RT-PCR. Each bar represents the mean ± SE of three independent experiments. D: shRNA-ADAM17 cells have shown a decreased in Erk phosphorylation. The immunoblotting indicates the expression of total Erk (anti-Erk), phosphorylated Erk (anti-phospho Erk) and as loading control (anti-GAPDH). E: Phosphorylation levels were calculated by band intensity using ImageJ software (n = 2, Fisher’s exact test p = 0.0001).