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Figure 2 | Molecular Cancer

Figure 2

From: Pim-1 kinase is a target of miR-486-5p and eukaryotic translation initiation factor 4E, and plays a critical role in lung cancer

Figure 2

Effects of Pim-1 on cell growth and cell cycle progression in NSCLC cells in vitro . (A) A549 and H1299 cells were transfected with Pim-1 siRNA at a final concentration of 50nM. Pim-1 expression was detected using qRT-PCR (Top) and Western blot (Bottom) at 48 h posttransfection. (B) A549 and H1299 cells were transfected as in (A). In the indicated time periods (0-96 h) posttransfection, cell viability was evaluated using MTT assay. (C) Cells were stained using propidium iodide (PI) 48 h posttransfection and the cell cycle distribution was analyzed by FACS. (D) CyclinD1 and CDK4 protein expressions were detected in A549 and H1299 cells after transfection with si-Pim-1 by Western Blot assay. All data were obtained from three independent experiments and shown as mean ± SD and *P <0.05.

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Molecular Cancer

ISSN: 1476-4598