Figure 3

Effects of Pim-1 on migration of NSCLC cells in vitro . (A) A549 and H1299 cells were transfected with Pim-1 siRNA at a final concentration of 50nM. The ability of cell motility was evaluated in A549 and H1299 cells after transfection with si-Pim-1 for 48 h using wound scratch assay. A uniform scratch was made in each confluent layer culture, the extent of wound closure was monitored under microscope, and photographs were taken at 0 and 48 h. (B) Knockdown of Pim-1 reduces A549 cell migration in the “Transwell assay”. Cells that migrated to the bottom chamber containing serum-supplemented medium were stained with crystal violet, visualized under microscope, and photographed at 24 h and 48 h, respectively. (C) Average number of cells migrated to the bottom chamber in five fields was counted manually. Data are shown as mean ± SD. Similar results were obtained in three independent experiments. *P < 0.05.