Figure 5
Expression Patterns of HE4 and ANXA2 in ovarian tissues. A, Cells at a density of 1 × 106 in 200 μl were injected into nude mice through the tail vein. Macroscopic view of lung metastatic nodes after inoculation with cancer cells. B, H&E staining in lung tissues of nude mice. High expression (a, original magnification × 200), low expression (b), and mock (c) groups are shown. C, Immunohistochemical staining of lung tissues of nude mice. High expression (a1, a2; original magnification × 200), low expression (b1, b2), and mock (c1, c2) groups are shown. D, Representative images of the peritoneal metastasis results. High expression (a), low expression (b), and mock (c) groups are shown. E, Real time PCR results show the expression of MKNK2 and LAMB2 in ovarian cancer CaoV-3 cells. Lane 1, mock group; lane 2, HE4 low expression groups; lane 3, HE4 low expression cells treated with HE4 active protein; lane 4, HE4 high expression groups. F, Immunohistochemical staining of ovarian malignant tumors (serous tumors, 1 and 2; mucinous tumors, 3 and 4; borderline tumors, 5 and 6; benign tumors, 7 and 8); and normal ovarian tissues, 9 and 10). HE4 (1, 3, 5, 7 and 9) and ANXA2 (2, 4, 6, 8 and 10; original magnification × 200).