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Figure 1 | Molecular Cancer

Figure 1

From: MCRS1 overexpression, which is specifically inhibited by miR-129*, promotes the epithelial-mesenchymal transition and metastasis in non-small cell lung cancer

Figure 1

Results of the morphological and functional experiments in NSCLC cells after MCRS1 silencing. (a) Left: Morphological observation of EPLC-32 M1 cells using phase-contrast microscopy. Right: Images of F-actin stained EPLC-32 M1 cells. (b and c) The results of the matrigel invasion assays in EPLC-32 M1 and NCI-H292 cells with or without MCRS1 knockdown. The invaded cells are stained with crystal violet (b), and the fold change in the number of invading cells is given as the mean ± SD from three independent experiments (c). (d) Cell permeability assay of the EPLC-32 M1 and NCI-H292 cells with or without MCRS1 silencing. The values of HRP permeability are given as the mean ± SD. (e) Western blot analysis of ZO-1, Occludin, E-cadherin and Vimentin in EPLC-32 M1 and NCI-H292 cells with or without MCRS1 depletion. (f) Morphological changes in 16HBE cells treated by TGF-β (ΔTGF-β) after transient MCRS1 knockdown (ΔMCRS1). (g) Western blot analysis of MCRS1, E-cadherin,and Vimentin in 16HBE cells treated by TGF-β (ΔTGF-β) after transient MCRS1 knockdown (ΔMCRS1). Luc: cells without MCRS1 silencing; Msh3: cells with stable MCRS1 silencing; *P <0.05 (Student’s t-test).

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Molecular Cancer

ISSN: 1476-4598