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Figure 3 | Molecular Cancer

Figure 3

From: Nedd4-1 is an exceptional prognostic biomarker for gastric cardia adenocarcinoma and functionally associated with metastasis

Figure 3

Nedd4-1 is essential for gastric cancer cell invasion and migration. A, Nedd4-1 in gastric cancer AGS and N87 cells was depleted by lentiviral vector-loaded Nedd4-1 shRNA. The gastric cancer cells were infected by lentiviral vector-loaded Nedd4-1 shRNA for 48 hours and endogenous Nedd4-1 in the cell lysates was detected by immunoblotting with anti-Nedd4-1. B, The effect of Nedd4-1 knockdown on AGS and N87 cell proliferation. C, The wound healing assay of the effect of Nedd4-1 knockdown on the gastric cancer cell migration. D, The invasion of AGS cells was determined by the transwell matrigel invasion assay. E, The migration of N87 cells was detected by the Transwell migration assay. The gastric cancer cell proliferation, migration and invasion assays were repeated three times and the results were consistent.

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