miR-940 expression in human prostate cancer and normal tissues . (A-B) In situ hybridization of the scrambled miR (miR-Scr) or miR-940 and H&E staining in normal/benign glands compared to the infiltrating tumor. (C) H&E staining (i), miR-940 (ii), and MIEN1 (iii) in cancer (top) and matched normal (bottom) tissues with miR-Scr (iv), and IgG control (v) representing the negative controls. (D) ~87% (n =13) tumor shows low (intensity ≤3) expression of miR-940 compared to ~80% (n =12) of the matched normal tissue with higher (intensity >3) miR-940 expression; conversely, ~13% (n =2) of the tumor expressed more (>3) miR-940 compared to lower expression (≤3) in ~20% (n =3) of the matched normal (n =15). Scale bar: 100μm.