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Figure 3 | Molecular Cancer

Figure 3

From: Sensitivity of leukemic T-cell lines to arsenic trioxide cytotoxicity is dependent on the induction of phosphatase B220/CD45R expression at the cell surface

Figure 3

Induction of phosphatase B220/CD45R and HSP70 on As 2 O 3 -treated T-cell lines. EL-4, BW5147, Jurkat, CD45-deficient Jurkat variant (J45.01) and HPB-ALL T-cell lines were treated with As2O3 for 24 h in doses ranging from 1 to 20 μM. (A) T-cell lines were then stained with PE-conjugated anti-B220/CD45R mAb or PE-conjugated rat IgG2a isotype control, and then analyzed by flow cytometry with respect to size (FSC) and B220 expression. FSC vs. B220 contour plots were used to determine the percentage of B220+ cells in region R1 (FSCint/lowSSChigh). At least 20,000 events were analyzed per sample. Squares in contour dots contain B220+ cells, and are drawn relative to the location of the cells in FSC vs. IgG2a isotype control contour plots. Numbers in the squares indicate the percentages of B220+ cells. Graphs report mean percentages ± SE (n =10 independent experiments) of B220+ cells versus the dose of As2O3. (B) T-cell lines were stained with APC-conjugated anti-B220 and FITC-conjugated anti-HSP70 antibodies or FITC-conjugated IgG and APC-conjugated IgG2a isotype control. FSC vs. SSC dot plots were used to define gates R1 and R2 with FSCint/lowSSChigh and FSChighSSClow, respectively. HSP70 vs. B220 dot plots were then gated in R1 and R2 to determine the percentages of cells expressing HSP70, B220 or both. At least 20,000 events were analyzed for each sample. Graphs report the percentages of cells co-expressing B220 and HSP70 in gates R1 () or R2 (■) versus the dose of As2O3.

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