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Figure 5 | Molecular Cancer

Figure 5

From: Sensitivity of leukemic T-cell lines to arsenic trioxide cytotoxicity is dependent on the induction of phosphatase B220/CD45R expression at the cell surface

Figure 5

Cell viability, cell morphology and B220/CD45R induction in T-cell lines stimulated with calcium ionophore A23187. Jurkat, HPB-ALL, L1210, EL-4 and BW5147 cells were treated with Calcium ionophore A23187 for 48 h in doses ranging from 100 to 800 nM. Cell viability measurement. Untreated and A23187-treated cells were stained with Annexin V and PI, and the percentages of living cells (Ann V- PI-) determined by flow cytometry. Graphs show mean percentages ± SE of Ann V- PI- from 4 independent experiments. Cell morphology analysis. Untreated and A23187-treated cells were analyzed by flow cytometry with respect to size (FSC) and granulosity (SSC). Regions R1 (◊) and R2 (■) identified on FSC vs. SSC dot plots encompassed cells with FSCint/lowSSChigh and FSChighSSClow, respectively. B220 induction. Untreated and A23187-treated T-cell lines were stained with anti-B220/CD45R mAb and analyzed by flow cytometry. Graphs report mean percentages ± SE (n = 4 independent experiments) of B220+ EL-4, BW5147, Jurkat and HPB-ALL cells at the indicated concentration of A23187. At least 20,000 events were analyzed per sample in all experiments reported in the graphs.

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Molecular Cancer

ISSN: 1476-4598