Figure 2

CUL4A regulates NSCLC cell growth both in vitro and in vivo . Ectopic and silencing CUL4A expression in H1299, H1650, A549 and H460 cells were established by viral transduction. The levels of CUL4A in these resultant cell lines were verified by RT-PCR (A) and Western blot (B). Cell proliferation in vitro was examined by MTT (C and D). Apoptosis was estimated using Annexin V staining as described in Methods (E and F). Tumorigenic capacity of A549 and A549-shCUL4A cells was assess in vivo (G, H, and I, n =6). ^*P <0.05 and ^**P <0.01 vs pBabe cells; ^#P <0.05 and ^##P <0.01 vs pSuper cells. All results in A to F are from three independent experiments. Error bar indicate standard deviation.