Figure 2
SIRT1 activation prevents oral cancer metastasis. (A) OSCC cells (105) were treated with 50 uM resveratrol (RSV; an SIRT1 agonist) and 10 uM sirtinol (an SIRT 1 antagonist) for 24 h, respectively. (B) Transient transfection of pEGFP-SIRT1 significantly inhibited the migration and invasion of OECM1 and HSC3 cells, which were rescued by siSIRT1. Transient transfected cells (overexpression-SIRT1 or knockdown SIRT1) were seeded in a 24-well chemotaxis chamber (1 × 104 cells/well) and incubated for 24 h with complete culture medium added in the lower chamber. Cell migration and invasion by Boyden chamber assays. Each data point represents the mean ± SD from at least three independent experiments. The asterisk indicates as statistically significant difference (*, p <0.05) compared to the pEGFP-C1 vector control or scrambled siRNA control.