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Figure 3 | Molecular Cancer

Figure 3

From: Role of SIRT1 in regulation of epithelial-to-mesenchymal transition in oral squamous cell carcinoma metastasis

Figure 3

Expression of epithelial and mesenchymal protein markers are regulated by SIRT1 in HOK and OSCC cells. (A) Western blotting revealed the expression levels of epithelial and mesenchymal protein markers in HOK and OSCC cell lines. Equal amounts of cell protein (20 ug) were immunoblotted with antibodies against SIRT1, E-cadherin, vimentin, N-cadherin, and β-actin. (B) Loss of SIRT1 increased expression of endogenous mesenchymal protein markers. Western blotting revealed expression of SIRT1 and EMT markers in HOK cells with or without siSIRT1. (C) Ectopic expression of SIRT1 increased expression of E-cadherin and reduced expression of vimentin and N-cadherin. Equal amounts of protein (20 ug) from OECM1 cells were transient transfected with pEGFP-SIRT1 or vector alone (pEGFP-C1) and analyzed by Western blot.

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Molecular Cancer

ISSN: 1476-4598