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Figure 4 | Molecular Cancer

Figure 4

From: Role of SIRT1 in regulation of epithelial-to-mesenchymal transition in oral squamous cell carcinoma metastasis

Figure 4

SIRT1 down-regulates expression and activity of MMP7. (A) qRT-PCR revealed the expression levels of MMP7 in OSCC cell lines after transient transfection with pEGFP-SIRT1 or vector alone (pEGFP-C1). Each data point represents the mean ± SD from at least three independent experiments. The asterisk indicates statistically significant difference (*, p <0.05) comparing to the control. (B) The MMP7 protein level was assessed by immunoblotting with anti-MMP7 antibody and GAPDH. MMP7 proteins in the cell homogenates were evaluated. (C) MMP7 activity was assayed by casein zymography. MMP7 activities in the cell media were compared between SIRT1-overexpressing or silencing OECM1 cells. (D) MMP7 concentrations and protein levels in OSCC cell media were assessed by ELISA and immunoblotting with anti-MMP7 antibody and GAPDH. Cell culture media were collected and concentrated from SIRT1-overexpressing or silencing OSCC cell lines.

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