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Figure 7 | Molecular Cancer

Figure 7

From: Role of SIRT1 in regulation of epithelial-to-mesenchymal transition in oral squamous cell carcinoma metastasis

Figure 7

SIRT1 represses the expression of MMP7 by deacetylating Smad4 in OSCCs. (A) Western blotting reveled the expression levels of Smad4, MMP7, and E-cadherin in OECM1 cell lines transient transfected with pEGFP-SIRT1 or vector alone (pEGFP-C1) for 24 h, and treated with TGF-β (5 ng/mL) for 48 h. (B) MMP7 activities of SIRT1-overexpressing or mock-transfected OECM1 cells were assayed by casein zymography after treatment with or without (TGF-β 5 ng/mL) for 48 h. (C) SIRT1 repressed expression of MMP7 by deacetylating K37 of Smad4. Western blotting revealed the expression and acetylation levels of endogenous Smad4 from flag-tagged Smad4 WT, Smad4-K37R, and Smad4-K428R OECM1 mutants with or without siSIRT1, and then purified by Flag beads. Western blots were probed with flag, acetylated-Lysine (Ac-K), and MMP7.

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