Figure 1
From: Exosome-mediated transfer of miR-10b promotes cell invasion in breast cancer
Characterization of exosome transfer. A, Electron micrograph of exosomes isolated from MDA-MB-231 conditioned medium, revealing the typical morphology and size (30-100 nm). B, Western blot showing presence of CD63 protein in exosome fraction from the same MDA-MB-231 conditioned medium. C, HEK-293T cells expressing CD63-GFP fusion protein compared to GFP alone. D, Cellular uptake of exosomes isolated from HEK-293T cells expressing the CD63-GFP fusion protein by MCF-10A cells. Image was captured 24 h after addition of exosomes to the culture. E, Intercellular trafficking of exosomes among different cell lines by isolated exosomes labeled with PKH26 dye. Shown here are for uptake in MCF-7 and HEK-293T cell lines. Images were taken 24 h after exosome addition by confocal microscope.