Figure 5
From: Exosome-mediated transfer of miR-10b promotes cell invasion in breast cancer
Exosomal miR-10b promotes cell invasion by targeting HOXD10. A, Exosomal miR-10b (E-miR-10b) suppresses the luciferase activity of the luciferase reporter carrying HOXD10 3′-UTR. Both wild-type (UTR-WT) or mutant (UTR-mut) reporters were introduced into HMLE cells by transfection and then incubated with the miR-10b-enriched exosomes or control. B, Suppression of HOXD10 protein in HMLE cells by exosomal miR-10b. Protein extract was prepared 48 h after addition of exosomal miR1-10b to the HMLE culture. C, Suppression of KLF4 in HEK-293T cells transfected with in KLF4 plus 3′-UTR. D, Suppression of cell invasion in HMLE cells by exosomal miR-10b. E, Quantitative analysis of data obtained from D.