Figure 2

Ectopic expression of SOX1 represses NPC cells proliferation and migration in vitro . (A) Ectopic expression of SOX1 in NPC cell lines was confirmed by WB (GAPDH as internal control) and IF. (B, C, D) Colony formation assay, cell proliferation assay and Ki67 staining were performed in NPC cells overexpressing SOX1. The colony formation ability reduced from 15.48  ±  3.29% to 4.90  ±  0.09% in CNE2 cells and from 11.14  ±  2.01% to 5.14  ±  0.82% in HONE1 cells. The Ki67 staining rate decreased from 0.54  ±  0.08 to 0.18  ±  0.05 in CNE2 cells and from 0.66  ±  0.11 to 0.29  ±  0.02 in HONE1 cells. Bar represents mean  ±  SD of three independent experiments (*p <0.05, **p <0.01, ***p <0.001, Student’s t test) (E, F) Wound-healing assay and transwell migration assay were performed in NPC cells overexpressing SOX1. The transwell migration cell number for each 20× field decreased from 64.33  ±  9.5 to 21.75  ±  2.99 in CNE2 cells and from 103.0  ±  18.2 to 27.0  ±  5.30 in HONE1 cells. Quantitative data are shown as the mean  ±  SD from three independent experiments. (*p <0.05, **p <0.01, ***p <0.001, Student’s t test). (Scale bars, 50 μm in A, D, 100 μm in E, F).