Figure 6
From: SOX1 down-regulates β-catenin and reverses malignant phenotype in nasopharyngeal carcinoma
SOX1 inhibits Wnt/β-catenin downstream genes and modulates the expression of molecules involved in the cell cycle. (A) WB for expression of c-Myc and Cyclin D1 in SOX1-overexpressing HONE1 cells. (B) Flow cytometry cell cycle analysis of HONE1 cells overexpressing SOX1. The percentage of cells in G1 phase increased from 67.77 ± 0.9% to 71.82 ± 1.05% while the percentage of cells in G2/M phase decreased from 12.7 ± 0.10% to 9.3 ± 0.10% in HONE1 cells overexpressing SOX1. Bar represents mean ± SD of three independent experiments. (*p <0.05, Student’s t test). (C) Proteins involved in the cell cycle after SOX1 expression was forced in HONE1 cells were identified via WB. GAPDH was used as an internal control.