SMURF1 inhibition reduces a CD44highcell population in SDCs. Representative flow cytometry analysis of CD44high cells in monolayer and in sphere-derived cells (SDCs) enriched from TR146 (A) and SCC-58 (B) sphere cultures are shown. A CD44high population is reduced in SMURF1-knockdown (shSMURF1) compared to shNeg and parental control SDCs after culturing in complete medium for 4 – 11 days. (C) Summary of flow cytometry analysis of CD44high TR146 SDCs shown in A (n = 5). (D) Summary of flow cytometry analysis of CD44high SCC-58 SDCs shown in B (n = 7). Data in C and D are presented as mean ± SE for the indicated number of independent repeats. Statistical analysis was performed using two-tailed Student’s t-test with equal variance. n.s. = not significant (p > 0.05).