Wnt/β-catenin signaling mediated miR-610-mediated HCC proliferation. A, Western blot analysis of LRP6 and TBL1X expression in miR-610-inhibited HCC cells transfected with LRP6-siRNA or TBL1X-siRNA. B, Luciferase assay of TCF/LEF transcriptional activity in indicated cells. C, Quantification of indicated HCC cell colonies determined by colony formation assay. D, Quantification of colony formation determined by anchorage-independent growth assay. E, Real-time PCR analysis of mRNA expression of CCND1 and MYC. Bars represent the means ± SD of three independent experiments. *P <0.05.