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Figure 1 | Molecular Cancer

Figure 1

From: Loss of vinculin and membrane-bound β-catenin promotes metastasis and predicts poor prognosis in colorectal cancer

Figure 1

Expression of vinculin in CRC cell lines and tissue samples. (A) The expression level of vinculin in five CRC cell lines and HIEC cells was measured by western blotting and quantified using Quantity One v4.6.2 software (one of three similar blots is shown). β-actin was used as a loading control. (B) The mRNA level of vinculin in five CRC cell lines and HIEC cells was measured by qRT–PCR using GAPDH as an internal control. (C) The expression of vinculin in adjacent non-cancerous colon tissues (N), primary CRC tissues (C) and lymph node metastatic tissues (M) from 6 patients was examined by western blotting. (D) The mRNA level of vinculin in paired tissues was measured using qRT-PCR. Vinculin expression was determined in tumour tissue relative to the patient’s adjacent normal tissue, and the relative expression of vinculin in the CRC cell lines was normalised to that in HIEC cells. Each sample was analysed in triplicate (*P < 0.05, **P < 0.01).

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Molecular Cancer

ISSN: 1476-4598