Figure 3
From: Ranolazine inhibits NaV1.5-mediated breast cancer cell invasiveness and lung colonization
Na V 1.5 suppression, or ranolazine treatment, inhibit metastatic lung colonisation by breast cancer cells. A, Representative bioluminescent imaging (BLI) measurement performed in the same NMRI nude mouse per condition from week 2 to week 8 after cancer cell injection. Mice were injected with shCTL MDA-MB-231-Luc cells (shCTL), or with shNaV1.5 MDA-MB-231-Luc cells (shNaV1.5) or with shCTL MDA-MB-231-Luc cells and treated (5 days/week) with ranolazine (50 mg/kg) (Rano) or vehicle (shCTL, shNaV1.5). B, Evolution of mice body weight during the experiments in the same conditions than in A. C, Mean in vivo BLI value (expressed in cpm) as a function of time recorded in the whole body of mice coming from the three groups indicated previously (shCTL, n = 18; shNaV1.5, n = 12; Rano, n = 8) (Statistical significance is indicated as: *p <0.05, Kruskal-Wallis analysis followed by Dunn’s test). D, Representative BLI at completion of the study (8th week after cells injection), in whole animals and ex vivo after lung isolation. E, BLI quantification of excised lungs. Box plots indicate the first quartile, the median, and the third quartile, squares indicate the mean (shCTL, n = 18; shNaV1.5, n = 12; Rano, n = 8) (Kruskal-Wallis analysis followed by Dunn’s test).