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Figure 1 | Molecular Cancer

Figure 1

From: RNAi-mediated silencing of MLL-AF9 reveals leukemia-associated downstream targets and processes

Figure 1

SiRNA knockdown of MLL-AF9 in THP1 cells. (A) Alignments of siRNAs to MLL-AF9 mRNA. Bold sequence represents the MLL part of the fusion transcript. (B) Confirmation of MLL-AF9 knockdown via RT-qPCR. Mean relative MLL-AF9 and HOXA9 transcript levels of knockdown (siRNA-A, siRNA-B) as compared to control treatments (control siRNA-1, -2) during the time course of experiments. Graph represents data from five independent experiments. Bars indicate standard deviation. (C) Immunoblot of MLL-AF9 from total protein extracted from day 8 experimental samples. Normalized MLL-AF9 levels are indicated at the bottom.

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