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Figure 1 | Molecular Cancer

Figure 1

From: Histone acetyltransferase-deficient p300 mutants in diffuse large B cell lymphoma have altered transcriptional regulatory activities and are required for optimal cell growth

Figure 1

Expression of p300 proteins in DLBCL cell lines. (a) Anti-p300 Western blotting was performed on whole-cell extracts from the indicated DLBCL cell lines. The sources of the cell lines are described in Materials and methods and in the indicated references in the figure. Thirty-five μg of total protein from whole-cell extracts was loaded for all samples except for RC-K8 (50 μg). (b) SUDHL2 cells express a C-terminally truncated p300 protein (p300ΔC-820). Whole-cell extracts from the six indicated cell lines were analyzed by Western blotting for relative expression of p300. The amount of total cell protein loaded is indicated. Western blots were performed with N-terminal anti-p300 antiserum. p300 expression was quantified by densitometric analysis and is relative to full-length p300 in Ramos cells (100). Relative p300 expression for the SUDHL2 and RC-K8 cell lines was determined by analyzing the bands corresponding to p300ΔC-820 and p300ΔC-1087, respectively. Full-length p300 in SUDHL2 and RC-K8 cell lines was undetectable (0). (c) Whole-cell extracts from the six indicated DLBCL cell lines were analyzed by Western blotting for expression of CBP. Twenty-five μg of whole-cell extract was used for each sample. Western blotting was performed with anti-CBP and anti-β-tubulin (as a loading control) antisera.

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Molecular Cancer

ISSN: 1476-4598