Figure 1

ZNF300P1 methylation patterns in EOC. (A) MeDIP-Chip methylation tracks (vertical bars denote methylation score of 1–5) of A2780 (red) and CaOV3 (purple) EOC cell lines compared to grouped profiles of three OSE (green) mapped to chromosome 5q33.1. Features of the chromosomal region are also shown including strand specific genes (black), CpG islands (dark green) and the Sequenom assay designed to interrogate promoter methylation (blue). (B) Average promoter methylation (% methylation as heatmap) of CpG islands flanking ZNF300P1 in EOC cell lines and immortalized OSE. Genes are sorted 5′ to 3′ on chromosome 5q33.1, cell lines are organized by similarity in methylation patterns. (C) Boxplot comparing average promoter methylation of ZNF300P1 in the eight clear cell tumors compared to 19 OSE. The p-value is generated by a Mann–Whitney U test.