Figure 2

ZNF300P1 cellular localization and phenotypic changes. (A) MALAT1 and GAPDH are included as nuclear and cytoplasmic transcript controls, respectively. Data is presented as mean of 3 independent experiments ± S.D. The effect of ZNF300P1 knockdown on HOSE17.1 cells. (B) Representative population growth of HOSE17.1 cells following transfection with non-targeting control (HOSE17.1-siNTC; dark grey) or siRNA against ZNF300P1 (HOSE17.1-siZP1; light grey). A line of best fit is shown for both conditions. (C) Representative colony formation at 10 days post-transfection. Each data point represents total number of colonies per 4 wells. (D) Windrose plots of cell migration patterns following an in vitro wound healing assay. Cell starting position is designated as 0,0 and the path taken over 24 hours is measured every 10 minutes (μm). (E) Boxplot of persistent migration ratios for individual cells from a representative experiment. Persistence was defined as the ratio of the total path length divided by the Euclidean displacement. P-values are generated by a Mann–Whitney U test.