siRNA-mediated down-regulation of WNK1/OSR1 reduces microchemotaxis of GCs. A. After 48 h treatment with either scramble siRNA (Scr), WNK1 siRNA, or OSR1 siRNA, serum-induced microchemotaxis of GC#22 was determined for 5 h under different treatment conditions control medium (Con), 10 μM BMT, 100 μM TMZ, or 100 μM TMZ plus 10 μM BMT (T + B). Representative images of GC#22 that have migrated through an 8-μm transwell barrier after 5 h were shown. B. Summary data of numbers of migrated GC#22 and GC#99 cells in different treatment groups. Data are mean ± SEM. n = 4, *p < 0.05 vs. Con. #p < 0.05 vs. Scr.