Skip to main content
Figure 1 | Molecular Cancer

Figure 1

From: Stable SET knockdown in head and neck squamous cell carcinoma promotes cell invasion and the mesenchymal-like phenotype in vitro, as well as necrosis, cisplatin sensitivity and lymph node metastasis in xenograft tumor models

Figure 1

ERK and p53 tumor-related protein levels, PP2A phosphatase activity, and proliferation and cell cycle analyses of the HNSCC shSET cells. (A) Western blotting was performed on HN12 cells using antibodies against SET, pERK1/2, total ERK1/2 and PP2Ac; tubulin was used as a constitutively expressed protein. (B) PP2A phosphatase activity was determined in HN12 cells using the Ser/Thr Phosphatase Assay system in the presence or absence of the PP2A inhibitor okadaic acid (OA). (C) Cell proliferation was assessed in HN12 cells using the BrdU incorporation assay. (D) Western blotting was performed in HN12 cells using antibodies against p53, p-p53Ser15 and p21; β-actin was used as a constitutively expressed protein. (E) Cell cycle distribution was assessed by PI/flow cytometry in HN12 and Cal27 cells. The results are representative of three independent experiments. For B and C, the reported values are the mean and standard deviation of experiments performed in triplicate (*p < 0.05; **p < 0.01). For A and D, the reported values are the shSET/shControl ratio, as described in the Methods section.

Back to article page

Molecular Cancer

ISSN: 1476-4598