Figure 3From: Stable SET knockdown in head and neck squamous cell carcinoma promotes cell invasion and the mesenchymal-like phenotype in vitro, as well as necrosis, cisplatin sensitivity and lymph node metastasis in xenograft tumor modelsMetalloproteinase (MMP-9 and MMP-2) activity as well as cofilin and F-actin expression in the HNSCC shSET cells. (A) Zymogram analysis using MMP secreted from HN12shSET cells at 24 and 72 h. (B) Zymogram analysis was performed in the presence of 1 mM APMA for 1 h at 37°C. The values indicated below the bands represent the shSET/shControl ratio of MMP activity. (C) Immunofluorescence analysis of F-actin (red) and cofilin (green), demonstrating the expression and distribution pattern of F-actin and cofilin; nuclei were stained with DAPI (blue). Arrows indicate cofilin aggregates in the cytoplasm. The results are representative of three independent experiments.Back to article page