Skip to main content
Figure 6 | Molecular Cancer

Figure 6

From: cAMP signaling inhibits radiation-induced ATM phosphorylation leading to the augmentation of apoptosis in human lung cancer cells

Figure 6

Agonists for Gαs-coupled receptors inhibited ATM phosphorylation and augmented apoptosis following γ-ray irradiation in H1299 cells. (A) Effects of prostaglandin E2 (PGE2) and isoproterenol (ISO) on PP2A B56δ and ATM phosphorylation. (B) Time-course of NF-κB luciferase activity after prostaglandin E2 or isoproterenol treatment. (C) Effects of prostaglandin E2 and isoproterenol on the cleavage of caspase 3 and PARP. (D) Effects of prostaglandin E2 and isoproterenol on early apoptosis. H1299 cells were treated with 1 μM isoproterenol or 10 μM PGE2 for 30 min before irradiation with γ-rays (5 Gy for or 10 Gy for others). Cells were then incubated for 30 min for analysis of phosphorylated ATM, B56δ, and CREB or 24 h for apoptosis analysis. For NF-κB luciferase activity assays, cells transfected with the reporter genes were treated with 1 μM isoproterenol or 10 μM PGE2 at30 min before irradiation (10 Gy), and the luciferase activity was measured at the indicated times (empty bar: control cells, filled bar: PGE2-treated cells, diagonal bar: isoproterenol-treated cells).

Back to article page