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Figure 1 | Molecular Cancer

Figure 1

From: Targeting the prohibitin scaffold-CRAF kinase interaction in RAS-ERK-driven pancreatic ductal adenocarcinoma

Figure 1

Expression of PHB in pancreatic cancer cells and tissue. A. Morphology of AsPC-1 and Capan-2 cells. Scale bar, 25 μm. B. RT-PCR analysis of PHB mRNA expression in AsPC-1 and Capan-2 cells. β-actin was used as a control. C. Immunoblot analysis of PHB protein in AsPC-1 and Capan-2 cells. α-tubulin was used as a control. D. Confocal microscopic images of the localization of PHB (red) in AsPC-1 and Capan-2 Cells. Nuclei were counterstained with DAPI. Scale bars, 25 μm. E. Quantification of the fluorescence intensity in (D). Values are the means ± SD of triplicate samples, *P < 0.01. F. Representative images of PHB expression in PDAC and normal epithelial tissues. Scale bar, 50 μm. G. Quantification of PHB expression in PDAC and normal pancreas tissues. Tumors were blindly scored based on the strength of PHB staining (n = 11 normal tissue samples and n = 46 PDAC tissue samples). Results are representative of three independent experiments.

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