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Figure 2 | Molecular Cancer

Figure 2

From: GPCR-like signaling mediated by smoothened contributes to acquired chemoresistance through activating Gli

Figure 2

Gαi and Gβγ are involved in mediating the Hh pathway activity in chemoresistant cancer cells. The data are expressed as the means ± s.d. from three independent experiments. A, Cells as indicated were transiently transfected with Gli luciferase reporter and pRL-Renilla luciferase plasmids, followed by treatment with PTX (100 ng/ml) for 24 h. The data are expressed as fold induction relative to K562 cells. B, Cells as indicated were transiently transfected with Gli luciferase reporter and pRL-Renilla luciferase plasmids together with GFP or Gαt. The cells were harvested for dual luciferase reporter assay after transfection for 48 h. C, KB/VCR cells after transfected with Gli luciferase reporter and pRL-Renilla luciferase plasmids were treated with SAG (100 ng/ml) with or without PTX (100 ng/ml) in culture medium with 0.5% serum for 24 h. D, KB/VCR cells after transfected with Gli luciferase reporter and pRL-Renilla luciferase plasmids supplemented with GFP or Gαt were stimulated with SAG in culture medium with 0.5% serum for 24 h. E, NIH-3T3 cells were transfected with Gli luciferase reporter and pRL-Renilla luciferase plasmids, and were treated with SAG (100 ng/ml) with or without PTX (100 ng/ml) in culture medium with 0.5% serum for 24 h. F, NIH-3T3 cells after transfected with Gli luciferase reporter and pRL-Renilla luciferase plasmids supplemented with GFP or Gαt were stimulated with SAG in culture medium with 0.5% serum for 24 h and were collected for dual lucifearse assay. G, NIH-3T3 cells were transfected with Gli luciferase reporter and pRL-Renilla luciferase plasmids supplemented with GFP, and Gβ1 together with Gγ2 respectively. H-I, Interference with the functions of Gαi and Gβγ by treatment with PTX (H) and transfection with Gαt (I) increases the sensitivity of KB/VCR (H) and K562/A02 (I) to chemotherapeutic drugs. J-K, Interference with the functions of Gαi and Gβγ by treatment with PTX (J) and transfection with Gαt (K) reduces the Gli1 expressions at mRNA level in KB/VCR cells and K562/A02 cells.

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