Figure 2From: GPCR-like signaling mediated by smoothened contributes to acquired chemoresistance through activating GliGαi and Gβγ are involved in mediating the Hh pathway activity in chemoresistant cancer cells. The data are expressed as the means ± s.d. from three independent experiments. A, Cells as indicated were transiently transfected with Gli luciferase reporter and pRL-Renilla luciferase plasmids, followed by treatment with PTX (100 ng/ml) for 24 h. The data are expressed as fold induction relative to K562 cells. B, Cells as indicated were transiently transfected with Gli luciferase reporter and pRL-Renilla luciferase plasmids together with GFP or Gαt. The cells were harvested for dual luciferase reporter assay after transfection for 48 h. C, KB/VCR cells after transfected with Gli luciferase reporter and pRL-Renilla luciferase plasmids were treated with SAG (100 ng/ml) with or without PTX (100 ng/ml) in culture medium with 0.5% serum for 24 h. D, KB/VCR cells after transfected with Gli luciferase reporter and pRL-Renilla luciferase plasmids supplemented with GFP or Gαt were stimulated with SAG in culture medium with 0.5% serum for 24 h. E, NIH-3T3 cells were transfected with Gli luciferase reporter and pRL-Renilla luciferase plasmids, and were treated with SAG (100 ng/ml) with or without PTX (100 ng/ml) in culture medium with 0.5% serum for 24 h. F, NIH-3T3 cells after transfected with Gli luciferase reporter and pRL-Renilla luciferase plasmids supplemented with GFP or Gαt were stimulated with SAG in culture medium with 0.5% serum for 24 h and were collected for dual lucifearse assay. G, NIH-3T3 cells were transfected with Gli luciferase reporter and pRL-Renilla luciferase plasmids supplemented with GFP, and Gβ1 together with Gγ2 respectively. H-I, Interference with the functions of Gαi and Gβγ by treatment with PTX (H) and transfection with Gαt (I) increases the sensitivity of KB/VCR (H) and K562/A02 (I) to chemotherapeutic drugs. J-K, Interference with the functions of Gαi and Gβγ by treatment with PTX (J) and transfection with Gαt (K) reduces the Gli1 expressions at mRNA level in KB/VCR cells and K562/A02 cells.Back to article page