Figure 3

Gαi and Gβγ are involved in the chemoresistance of KB cells with forced expression of SmoA1 by activating Gli. KB cells were infected with lenti-virus harboring GFP, Flag-tagged SmoA1, and Flag-tagged SmoA1 plus Gαt with a HA tag. A, Western blot analysis of p-JNK, Flag tagged SmoA1 and HA tagged Gαt. B, SmoA1 causes KB cells insensitive to VCR, while PTX (100 ng/ml) treatment recovers the sensitivity of KB cells with forced expression of SmoA1 to VCR. Data are expressed as average ± s.d. from three separate experiments. C, SmoA1 enhances the expression of Gli1 at mRNA level, while PTX treatment for 24 h abolishes this enhancement of Gli1 expression at mRNA level. The results are expressed as the means ± s.d. from three to four independent experiments. D, SmoA1 causes KB cells insensitive to VCR, while co-expression of Gαt with SmoA1 by lenti-virus approach circumvents this tolerance to VCR. Data are expressed as average ± s.d. from three separate experiments. E, SmoA1 increases the expression of Gli1 at mRNA level in KB cells compared to GFP cohort, while co-expression of Gαt with SmoA1 reduces the expression of Gli1. The results are expressed as the means ± s.d. from three to four independent experiments.