Figure 5

miR-425 inhibits cell apoptosis by repressing PTEN. (A). AGS cells were treated with control (PBS) or IL-1β. After 48 h, whole cell lysates were immunoblotted. (B). AGS cells were transiently transfected with negative control (miR-NC) or miR-425 alone. After 24 h, the cells were treated with cisplatin and harvested 24 h after treatment. Whole cell lysates were immunoblotted. (C). AGS cells were transfected with miR-NC, miR-425, and PTEN-CDS as indicated. After 24 h, the cells were treated with cisplatin and harvested 24 h after treatment. The cell apoptosis rate was determined using the flow cytometry method. (D). AGS cells were transfected with miR-NC or anti-miR-425. After 48 h, the cells were treated with IL-1β and harvested 24 h after treatment. Total cell lysates were immunoblotted with the indicated antibodies, and the cell apoptosis rate was determined using the flow cytometry method. (E) NCI-N87 cells were transfected with miR-NC or anti-miR-425. Total cell lysates were immunoblotted with the indicated antibodies, and the cell apoptosis rate was determined using the flow cytometry method.