Figure 6

miR-425 promotes cell proliferation by repressing PTEN. (A) AGS cells were treated with PBS, IL-1β, miR-NC, miR-425, and PTEN-CDS as indicated. After 72 h, cell proliferation was determined using the EdU labeling kit. (B) NCI-N87 cells were transfected with miR-NC or anti-miR-425. After 72 h, the cell proliferation rate was determined using the EdU labeling kit. (C) AGS cells were treated with siRNA-PTEN or miR-425. After 72 h, cell proliferation was determined using the EdU labeling kit. (D) Photographs of PTEN protein expression in the tumor tissues (upper panels) and tumors (lower panels). (E) The graph shows the weight of the 3 tumors after 4 weeks (n = 3). (F) A significant inverse correlation is observed between the miR-425 and PTEN expression levels in the gastric cancer tissues (n = 52). (G) The expression levels of PTEN are determined in six normal gastric mucosa cells and gastric cancer cell lines using real-time PCR. (H) A model illustrating the putative roles of IL-1β and miR-425 in the control of the PTEN pathway in human gastric carcinoma cells.