Figure 2

CaSR expression in primary RCC cells of different metastatic potential. CaSR expression was quantified in primary RCC cells of 9 patients developing no metastases, lung or bone metastases within 5 years after nephrectomy (3/category) after treatment with 5 mM calcium for 30 min by flow cytometry. Exemplarily demonstrated in the histograms are IgG control (gray line) and calcium treated cells (black line). CaSR expression was highest in patients with bone metastases, p = 0.006. Treatment with calcium (5 mM) had no effect on CaSR expression. The columns represent mean values of 10.000 counted cells and standard error in% of values of non-metastasizing tumor cells (= cells of patients without metastases). An isotype specific IgG control antibody was used for control.